Biology Lab Report

In: Science

Submitted By brittanybarnes
Words 2636
Pages 11
BIOL 1F90

Experiment #5 - Archaea: Characterization of Halophiles

Student Name: Brittany Barnes

Student Number: 5408224

Lab Section: 35

Date Experiment Performed: January 13, 2014

Lab Partners:
Kayla Hutchinson
Fallon Fersaud
Ashley Maulucci

Abstract

Halobacterium salinarum and Haloferax volcanii come from the domain called Archaea and are considered to be halophilic, because they thrive in extreme environments. In this lab experiment, several tasks took place. In order to be able to observe the colony morphology of both Archaea, they were grown on an agar surface that allowed them to form colonies of characteristic colour and appearance. In order to observe the physiology of both Archaea on the effect of salt concentration, pH, and temperature, they needed to be placed on agar plates and incubated for two weeks. Being incubated for two weeks, allowed the halophilic archaeal cultures to grow. The objective of this experiment was to determine the morphological and biochemical characteristics along with the growth requirements of the halophilic Archaeans; Halobacterium salinarum NRC-1 and Haloferax volcanii DS2.

Introduction

The domain, Archaea, possesses prokaryotic cells and has a cell wall that contains no peptidoglycan. Archaea contain rRNA that is unique to the Archaea as indicated by the presence of molecular regions. Archaea usually live in extreme environments and include methanogens, extreme halophiles, and hyperthermophiles. One reason for this is that the ether-containing linkages in the Archaea membranes are more stable and are able to withstand higher temperatures and stronger acid concentrations. The other two domains of life are Bacteria and Eukarya. Unlike the Bacteria and the Eukarya domains, the Archaea domain has membranes composed of branched hydrocarbon chains that are attached to glycerol by ether…...

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