Nitrate Levels

In: Science

Submitted By jlive12
Words 923
Pages 4
Nitrate levels have an effect on the chlorophyll levels in Campus Lake

Abstract

Algae Chlamydomonas is an example of a photosynthetic bacteria that produces chlorophyll in Campus Lake. Temperature can increase and decrease the activeness of the photosynthetic bacteria. Nitrate levels and chlorophyll levels were recorded for four seasons. Nitrate and chlorophyll levels were highest in the summer and lowest in the winter. The temperature was also highest in the summer and lowest in the winter. Nitrate levels impact the chlorophyll levels in Campus Lake.

Introduction

The purpose of this study was to determine if nitrate levels impacted the chlorophyll levels in Campus Lake from Spring 2009 – Winter 2010. This time frame was selected, because it was a full calendar year with all four seasons. Nitrate, NO3, is the form of nitrogen that can be used by plants to convert to nitrogen for heterotrophs. Chlorophyll is found in autotrophs and can be used as a relative measure of autotrophic concentration (Wischusen, 2009). It was hypothesized that the nitrate levels will positively impact the chlorophyll levels in Campus Lake. If the hypothesis is true, as nitrate levels increase, chlorophyll levels will also increase.

Materials and Methods

Measurement of nitrate production was performed using sample water from Campus Lake, NitraVer3 Reagent, and NitraVer6 Reagent. 5 mL of sample water was put in a test tube and NitraVer6 Reagent was added to it. The test tube was shook for 3 minutes and incubated for 30 seconds. After incubation, the sample was poured into a viewing tube but was poured very carefully, so no settled cadmium particles transferred to the viewing tube. NitraVer3 Reagent was added to the same viewing tube with the sample. After adding the second reagent, the tube was capped, shook for 30 seconds, and incubated for 10 minutes. A red color…...

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